Publications

PUBLICATIONS

2018


Mortensen SA, Sander B, Jensen RK, Pedersen JS, Golas MM, Thiel S, Andersen
GR. Models of the complement C1 complex. Proc Natl Acad Sci U S A. 2018 Apr 13.
Epub ahead of print.

By mistake, the wrong files were deposited in SASBDB. This was corrected in April 2018, and the correct files are also available HERE

Jensen RK, Pihl R, Gadeberg TAF, Jensen JK, Andersen KR, Thiel S, Laursen NS, Andersen GR. A potent complement factor C3 specific nanobody inhibiting multiple functions in the alternative pathway of human and murine complement. J Biol Chem. 2018 Mar 1. Epub ahead of print

Jabs F, Plum M, Laursen NS, Jensen RK, Mølgaard B, Miehe M, Mandolesi M, Rauber MM, Pfützner W, Jakob T, Möbs C, Andersen GR, Spillner E. Trapping IgE in a closed conformation by mimicking CD23 binding prevents and disrupts FcεRI interaction. Nat Commun. 2018 Jan 2;9(1):7.

 

2017


Hansen SB, Laursen NS, Andersen GR, Andersen KR. Introducing site-specific cysteines into nanobodies for mercury labelling allows de novo phasing of their crystal structures. Acta Crystallogr D Struct Biol. 2017 Oct 1;73(Pt 10):804-813.

Jank T, Belyi Y, Wirth C, Rospert S, Hu Z, Dengjel J, Tzivelekidis T, Andersen GR, Hunte C, Schlosser A, Aktories K. Protein glutaminylation is a yeast-specific posttranslational modification of elongation factor 1A. J Biol Chem. 2017 Sep 29;292(39):16014-16023.

Mortensen SA, Sander B, Jensen RK, Pedersen JS, Golas M, Jensenius JC, Hansen AG, Thiel S, Andersen GR. Reply to Arlaud et al.: Structure of the C1 complex and the unbound C1r2s2 tetramer. Proc Natl Acad Sci U S A. 2017 Jul 18;114(29)

He Y, Staley JP, Andersen GR, Nielsen KH. Structure of the DEAH/RHA ATPase Prp43p bound to RNA implicates a pair of hairpins and motif Va in translocation along RNA. RNA. 2017 Jul;23(7):1110-1124

Pedersen DV, Roumenina L, Jensen RK, Gadeberg TA, Marinozzi C, Picard C, Rybkine T, Thiel S, Sørensen UB, Stover C, Fremeaux-Bacchi V, Andersen GR. Functional and structural insight into properdin control of complement alternative pathway amplification. EMBO J. 2017 Apr 13;36(8):1084-1099.

Mortensen SA, Sander B, Jensen RK, Pedersen JS, Golas MM, Jensenius JC, Hansen AG, Thiel S, Andersen GR. Structure and activation of C1, the complex initiating the classical pathway of the complement cascade. Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):986-991.

By mistake, the wrong files were deposited in SASBDB. This was corrected in April 2018, and the correct files are also available HERE

 

2016


Yatime L, Betzer C, Jensen RK, Mortensen S, Jensen PH, Andersen GR. The Structure of the RAGE:S100A6 Complex Reveals a Unique Mode of Homodimerization for S100 Proteins. Structure. 2016 Dec 6;24(12):2043-2052.

Schatz-Jakobsen JA, Pedersen DV, Andersen GR. Structural insight into proteolytic activation and regulation of the complement system. Immunol Rev. 2016 Nov;274(1):59-73.

Croll TI, Andersen GR. Re-evaluation of low-resolution crystal structures via interactive molecular-dynamics flexible fitting (iMDFF): a case study in complement C4. Acta Crystallogr D Struct Biol. 2016 Sep;72(Pt 9):1006-16.

Jensen MR, Bajic G, Zhang X, Laustsen AK, Koldsø H, Skeby KK, Schiøtt B, Andersen GR, Vorup-Jensen T. Structural Basis for Simvastatin Competitive Antagonism of Complement Receptor 3. J Biol Chem. 2016 Aug 12;291(33):16963-76.

Mortensen S, Jensen JK, Andersen GR. Solution Structures of Complement C2 and Its C4 Complexes Propose Pathway-specific Mechanisms for Control and Activation of the Complement Proconvertases. J Biol Chem. 2016 Aug 5;291(32):16494-507.

Lin H, Andersen GR, Yatime L. Crystal structure of human S100A8 in complex with zinc and calcium. BMC Struct Biol. 2016 Jun 1;16(1):8.

Schatz-Jakobsen JA, Zhang Y, Johnson K, Neill A, Sheridan D, Andersen GR. Structural Basis for Eculizumab-Mediated Inhibition of the Complement Terminal Pathway. J Immunol. 2016 Jul 1;197(1):337-44.

Kidmose RT, Andersen GR. Interacting with the Human Insulin Receptor. Structure. 2016 Mar 1;24(3):351-2.

Zhang X, Bajic G, Andersen GR, Christiansen SH, Vorup-Jensen T. The cationic peptide LL-37 binds Mac-1 (CD11b/CD18) with a low dissociation rate and promotes phagocytosis. Biochim Biophys Acta. 2016 May;1864(5):471-8.

 

2015


Bajic G, Degn SE, Thiel S, Andersen GR. Complement activation, regulation, and molecular basis for complement-related diseases. EMBO J. 2015 Nov 12;34(22):2735-57.

Stevenson L, Laursen E, Cowan GJ, Bandoh B, Barfod L, Cavanagh DR, Andersen GR, Hviid L. α2-Macroglobulin Can Crosslink Multiple Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) Molecules and May Facilitate Adhesion of Parasitized Erythrocytes. PLoS Pathog. 2015 Jul 2;11(7):e1005022.

Mortensen S, Kidmose RT, Petersen SV, Szilágyi Á, Prohászka Z, Andersen GR. Structural Basis for the Function of Complement Component C4 within the Classical and Lectin Pathways of Complement. J Immunol. 2015 Jun 1;194(11):5488-96.

Yatime L, Maasch C, Hoehlig K, Klussmann S, Andersen GR, Vater A. Structural basis for the targeting of complement anaphylatoxin C5a using a mixed L-RNA/L-DNA aptamer. Nat Commun. 2015 Apr 22;6:6481.

Andersen GR. Mutations in complement C3 from aHUS patients. Blood. 2015 Apr 9;125(15):2316-8.

Jensen RK, Plum M, Tjerrild L, Jakob T, Spillner E, Andersen GR. Structure of the omalizumab Fab. Acta Crystallogr F Struct Biol Commun. 2015 Apr;71(Pt 4):419-26.

Kläning E, Christensen B, Bajic G, Hoffmann SV, Jones NC, Callesen MM, Andersen GR, Sørensen ES, Vorup-Jensen T. Multiple low-affinity interactions support binding of human osteopontin to integrin αXβ2. Biochim Biophys Acta. 2015 Aug;1854(8):930-8.

Kjaer TR, Le le TM, Pedersen JS, Sander B, Golas MM, Jensenius JC, Andersen GR, Thiel S. Structural insights into the initiating complex of the lectin pathway of complement activation. Structure. 2015 Feb 3;23(2):342-51.

 

2014


Degn SE, Kjaer TR, Kidmose RT, Jensen L, Hansen AG, Tekin M, Jensenius JC, Andersen GR, Thiel S. Complement activation by ligand-driven juxtaposition of discrete pattern recognition complexes. Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13445-50.

Schatz-Jakobsen JA, Yatime L, Larsen C, Petersen SV, Klos A, Andersen GR. Structural and functional characterization of human and murine C5a anaphylatoxins. Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1704-17.

Yatime L, Andersen GR. The specificity of DNA recognition by the RAGE receptor. J Exp Med. 2014 May 5;211(5):749-50

Favaro FP, Alvizi L, Zechi-Ceide RM, Bertola D, Felix TM, de Souza J, Raskin S, Twigg SR, Weiner AM, Armas P, Margarit E, Calcaterra NB, Andersen GR, McGowan SJ, Wilkie AO, Richieri-Costa A, de Almeida ML, Passos-Bueno MR. A noncoding expansion in EIF4A3 causes Richieri-Costa-Pereira syndrome, a craniofacial disorder associated with limb defects. Am J Hum Genet. 2014 Jan 2;94(1):120-8.

Sottrup-Jensen L, Andersen GR. Purification of human complement protein C5. Methods Mol Biol. 2014;1100:93-102.

 

2013


Yatime L, Andersen GR. Structural insights into the oligomerization mode of the human receptor for advanced glycation end-products. FEBS J. 2013 Dec;280(24):6556-68.

Bajic G, Yatime L, Sim RB, Vorup-Jensen T, Andersen GR. Structural insight on the recognition of surface-bound opsonins by the integrin I domain of complement receptor 3. Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16426-31.

Kjaer TR, Thiel S, Andersen GR. Toward a structure-based comprehension of the lectin pathway of complement. Mol Immunol. 2013 Dec;56(4):413-22.

Brodersen DE, Andersen GR, Andersen CB. Mimer: an automated spreadsheet-based crystallization screening system. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jul;69(Pt 7):815-20.

Bajic G, Yatime L, Klos A, Andersen GR. Human C3a and C3a desArg anaphylatoxins have conserved structures, in contrast to C5a and C5a desArg. Protein Sci. 2013 Feb;22(2):204-12.

 

2012


Kidmose RT, Laursen NS, Dobó J, Kjaer TR, Sirotkina S, Yatime L, Sottrup-Jensen L, Thiel S, Gál P, Andersen GR. Structural basis for activation of the complement system by component C4 cleavage. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15425-30.

Andersen CB, Torvund-Jensen M, Nielsen MJ, de Oliveira CL, Hersleth HP, Andersen NH, Pedersen JS, Andersen GR, Moestrup SK. Structure of the haptoglobin-haemoglobin complex. Nature. 2012 Sep 20;489(7416):456-9

Laursen NS, Magnani F, Gottfredsen RH, Petersen SV, Andersen GR. 2012, Structure, function and control of complement C5 and its proteolytic fragments. Curr Mol Med. Sep 1;12(8):1083-97

Behrens MA, He Y, Oliveira CL, Andersen GR, Pedersen JS, Nielsen KH. Structural analysis of RNA helicases with small-angle X-ray scattering. Methods Enzymol. 2012;511:191-212.

Marrero A, Duquerroy S, Trapani S, Goulas T, Guevara T, Andersen GR, Navaza J, Sottrup-Jensen L, Gomis-Rüth FX. The crystal structure of human α2-macroglobulin reveals a unique molecular cage. Angew Chem Int Ed Engl. 2012 Apr 2;51(14):3340-4.

 

2011


He Y, Andersen GR, Nielsen KH. The function and architecture of DEAH/RHA helicases. Biomol Concepts. 2011 Aug 1;2(4):315-26.

Laursen NS, Andersen KR, Braren I, Spillner E, Sottrup-Jensen L, Andersen GR. Substrate recognition by complement convertases revealed in the C5-cobra venom factor complex. EMBO J. 2011 Feb 2;30(3):606-16.

Nielsen KH, Behrens MA, He Y, Oliveira CL, Jensen LS, Hoffmann SV, Pedersen JS, Andersen GR. Synergistic activation of eIF4A by eIF4B and eIF4G. Nucleic Acids Res. 2011 Apr;39(7):2678-89.

 

2010


Kidmose RT, Vasiliev NN, Chetverin AB, Andersen GR, Knudsen CR. Structure of the Qβ replicase, an RNA-dependent RNA polymerase consisting of viral and host proteins. Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10884-9

Juul T, Malolepszy A, Dybkaer K, Kidmose R, Rasmussen JT, Andersen GR, Johnsen HE, Jørgensen JE, Andersen SU. The in vivo toxicity of hydroxyurea depends on its direct target catalase. J Biol Chem. 2010 Jul 9;285(28):21411-5.

Andersen CB, Madsen M, Storm T, Moestrup SK, Andersen GR. Structural basis for receptor recognition of vitamin-B(12)-intrinsic factor complexes. Nature. 2010 Mar 18;464(7287):445-8.

He Y, Andersen GR, Nielsen KH. Structural basis for the function of DEAH helicases. EMBO Rep. 2010 Mar;11(3):180-6.

Laursen NS, Gordon N, Hermans S, Lorenz N, Jackson N, Wines B, Spillner E, Christensen JB, Jensen M, Fredslund F, Bjerre M, Sottrup-Jensen L, Fraser JD, Andersen GR. Structural basis for inhibition of complement C5 by the SSL7 protein from Staphylococcus aureus. Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3681-6.

 

2009


Nielsen KH, Chamieh H, Andersen CB, Fredslund F, Hamborg K, Le Hir H, Andersen GR. Mechanism of ATP turnover inhibition in the EJC. RNA. 2009 Jan;15(1):67-75.

 

2008


Fredslund F, Laursen NS, Roversi P, Jenner L, Oliveira CL, Pedersen JS, Nunn MA, Lea SM, Discipio R, Sottrup-Jensen L, Andersen GR. Structure of and influence of a tick complement inhibitor on human complement component 5. Nat Immunol. 2008 Jul;9(7):753-60.

Le Hir H, Andersen GR. Structural insights into the exon junction complex. Curr Opin Struct Biol. 2008 Feb;18(1):112-9.

 

2007


Taylor DJ, Nilsson J, Merrill AR, Andersen GR, Nissen P, Frank J. Structures of modified eEF2 80S ribosome complexes reveal the role of GTP hydrolysis in translocation. EMBO J. 2007 May 2;26(9):2421-31.

Søe R, Mosley RT, Justice M, Nielsen-Kahn J, Shastry M, Merrill AR, Andersen GR. Sordarin derivatives induce a novel conformation of the yeast ribosome translocation factor eEF2. J Biol Chem. 2007 Jan 5;282(1):657-66.

 

2006


Andersen CB, Ballut L, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Séraphin B, Le Hir H, Andersen GR. Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA. Science. 2006 Sep 29;313(5795):1968-72.

Andersen CB, Becker T, Blau M, Anand M, Halic M, Balar B, Mielke T, Boesen T, Pedersen JS, Spahn CM, Kinzy TG, Andersen GR, Beckmann R. Structure of eEF3 and the mechanism of transfer RNA release from the E-site. Nature. 2006 Oct 12;443(7112):663-8.

Fredslund F, Jenner L, Husted LB, Nyborg J, Andersen GR, Sottrup-Jensen L. The structure of bovine complement component 3 reveals the basis for thioester function. J Mol Biol. 2006 Aug 4;361(1):115-27.

Pittman YR, Valente L, Jeppesen MG, Andersen GR, Patel S, Kinzy TG. Mg2+ and a key lysine modulate exchange activity of eukaryotic translation elongation factor 1Bα. J Biol Chem. 2006 Jul 14;281(28):19457-68.

Yates SP, Jørgensen R, Andersen GR, Merrill AR. Stealth and mimicry by deadly bacterial toxins. Trends Biochem Sci. 2006 Feb;31(2):123-33.

 

2005


Jørgensen R, Merrill AR, Yates SP, Marquez VE, Schwan AL, Boesen T, Andersen GR. Exotoxin A-eEF2 complex structure indicates ADP ribosylation by ribosome mimicry. Nature. 2005 Aug 18;436(7053):979-84.

Yates SP, Taylor PL, Jørgensen R, Ferraris D, Zhang J, Andersen GR, Merrill AR. Structure-function analysis of water-soluble inhibitors of the catalytic domain of exotoxin A from Pseudomonas aeruginosa. Biochem J. 2005 Feb 1;385(Pt 3):667-75.

 

2004


Jørgensen R, Yates SP, Teal DJ, Nilsson J, Prentice GA, Merrill AR, Andersen GR. Crystal structure of ADP-ribosylated ribosomal translocase from Saccharomyces cerevisiae. J Biol Chem. 2004 Oct 29;279(44):45919-25.

Andersen CF, Anand M, Boesen T, Van LB, Kinzy TG, Andersen GR. Purification and crystallization of the yeast translation elongation factor eEF3. Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1304-7.

Spahn CM, Gomez-Lorenzo MG, Grassucci RA, Jørgensen R, Andersen GR, Beckmann R, Penczek PA, Ballesta JP, Frank J. Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation. EMBO J. 2004 Mar 10;23(5):1008-19.

Boesen T, Mohammad SS, Pavitt GD, Andersen GR. Structure of the catalytic fragment of translation initiation factor 2B and identification of a critically important catalytic residue. J Biol Chem. 2004 Mar 12;279(11):10584-92.

 

2003


Jeppesen MG, Ortiz P, Shepard W, Kinzy TG, Nyborg J, Andersen GR. The crystal structure of the glutathione S-transferase-like domain of elongation factor 1Bγ from Saccharomyces cerevisiae. J Biol Chem. 2003 Nov 21;278(47):47190-8.

Andersen GR, Nissen P, Nyborg J. Elongation factors in protein biosynthesis. Trends Biochem Sci. 2003 Aug;28(8):434-41.

Vanwetswinkel S, Kriek J, Andersen GR, Güntert P, Dijk J, Canters GW, Siegal G. Solution structure of the 162 residue C-terminal domain of human elongation factor 1Bγ. J Biol Chem. 2003 Oct 31;278(44):43443-51.

Vanwetswinkel S, Kriek J, Andersen GR, Dijk J, Siegal G. 1H, 15N and 13C resonance assignments of the highly conserved 19 kDa C-terminal domain from human elongation factor 1Bγ. J Biomol NMR. 2003 Jun;26(2):189-90.

Jørgensen R, Ortiz PA, Carr-Schmid A, Nissen P, Kinzy TG, Andersen GR. Two crystal structures demonstrate large conformational changes in the eukaryotic ribosomal translocase. Nat Struct Biol. 2003 May;10(5):379-85.

 

2002


Karring H, Andersen GR, Thirup SS, Nyborg J, Spremulli LL, Clark BF. Isolation, crystallisation, and preliminary X-ray analysis of the bovine mitochondrial EF-Tu:GDP and EF-Tu:EF-Ts complexes. Biochim Biophys Acta. 2002 Dec 16;1601(2):172-7.

Jørgensen R, Carr-Schmid A, Ortiz PA, Kinzy TG, Andersen GR. Purification and crystallization of the yeast elongation factor eEF2. Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):712-5.

 

2001


Vestergaard B, Van LB, Andersen GR, Nyborg J, Buckingham RH, Kjeldgaard M. Bacterial polypeptide release factor RF2 is structurally distinct from eukaryotic eRF1. Mol Cell. 2001 Dec;8(6):1375-82.

Andersen GR, Valente L, Pedersen L, Kinzy TG, Nyborg J. Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Bα complex. Nat Struct Biol. 2001 Jun;8(6):531-4.

Pedersen L, Andersen GR, Knudsen CR, Kinzy TG, Nyborg J. Crystallization of the yeast elongation factor complex eEF1A-eEF1Bα. Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):159-61.

 

2000


Andersen GR, Pedersen L, Valente L, Chatterjee I, Kinzy TG, Kjeldgaard M, Nyborg J. Structural basis for nucleotide exchange and competition with tRNA in the yeast elongation factor complex eEF1A:eEF1Bα. Mol Cell. 2000 Nov;6(5):1261-6.

Andersen GR, Thirup S, Spremulli LL, Nyborg J. High resolution crystal structure of bovine mitochondrial EF-Tu in complex with GDP. J Mol Biol. 2000 Mar 24;297(2):421-36.

 

1999


Brodersen DE, Jenner LB, Andersen GR, Nyborg J. Xact: A program for construction, automated setup, and bookkeeping of crystallisation experiments. J Appl Crystallogr. 1999 Oct Vol. 32 (Pt 5): 1012-1016.

 

1997


Andersen G, Busso D, Poterszman A, Hwang JR, Wurtz JM, Ripp R, Thierry JC, Egly JM, Moras D. The structure of cyclin H: common mode of kinase activation and specific features. EMBO J. 1997 Mar 3;16(5):958-67.

Poterszman A, Andersen G, Busso D, Rossignol M, Egly JM, Thierry JC. Expression in Escherichia coli: purification and characterization of cyclin H, a subunit of the human general transcription/DNA repair factor TFIIH. Protein Expr Purif. 1997 Mar;9(2):153-8.

 

1996


Andersen G, Poterszman A, Egly JM, Moras D, Thierry JC. The crystal structure of human cyclin H. FEBS Lett. 1996 Nov 11;397(1):65-9.

 

1995


Andersen GR, Koch TJ, Dolmer K, Sottrup-Jensen L, Nyborg J. Low resolution X-ray structure of human methylamine-treated α2-macroglobulin. J Biol Chem. 1995 Oct 20;270(42):25133-41.

Dolmer K, Jenner LB, Jacobsen L, Andersen GR, Koch TJ, Thirup S, Sottrup-Jensen L, Nyborg J. Crystallisation and preliminary X-ray analysis of the receptor-binding domain of human and bovine α2-macroglobulin. FEBS Lett. 1995 Sep 18;372(1):93-5.

 

1994


Andersen GR, Koch T, Sørensen AH, Thirup S, Nyborg J, Dolmer K, Jacobsen L, Sottrup-Jensen L. Crystallization of proteins of the α2-macroglobulin superfamily. Ann N Y Acad Sci. 1994 Sep 10;737:444-6.

Sørensen AH, Dolmer K, Thirup S, Andersen GR, Sottrup-Jensen L, Nyborg J. Crystallization of human methylamine-treated complement C3 and C3b. Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):786-9.

Andersen GR. Low-resolution X-ray diffraction data obtained from hexagonal crystals of methylamine-treated α2-macroglobulin. Acta Crystallogr D Biol Crystallogr. 1994 May 1;50(Pt 3):298-301.

 

1991


Andersen GR, Jacobsen L, Thirup S, Nyborg J, Sottrup-Jensen L. Crystallization and preliminary X-ray analysis of methylamine-treated α2-macroglobulin and 3 α2-macroglobulin-proteinase complexes. FEBS Lett. 1991 Nov 4;292(1-2):267-70.