Lab members

Group leader

Professor Gregers Rom Andersen

Gregers obtained his PhD degree from the Department of Chemistry, Aarhus University in 1995. After his graduate studies Gregers performed a two-years postdoc in Strasbourg in the group of Professor Dino Moras, IGBMC, Université Louis Pasteur, working on structure determination of human cyclin H. In 1997 Gregers returned to Aarhus University, holding a position as Associate Professor at the Department of Molecular Biology and Genetics from 2002-2013, working on RNA helicases and the complement system. In 2009 Gregers was awarded a Hallas-Møller stipend by the Novo-Nordisk Foundation and in 2013 he was appointed Professor in Structural Immunology at the Department of Molecular Biology and Genetics. From 2007-2012, Gregers was chairman of the Danish National Committee for Crystallography and he is a member of EMBO since 2011. Gregers has a present focus on structural biology of the complement system as well as on branches of the adaptive immune system. His group is specialized in the use of X-ray crystallography in combination with small angle X-ray scattering (SAXS) and single particle electron microscopy (EM). Currently Gregers is partner in the BRAINSTRUC project funded by the Lundbeck Foundation, aiming at a structural description of selected brain functions and diseases.

 

Assistant Professor

Nick Stub Laursen

Nick obtained his PhD degree in Molecular Biology in 2011 from Aarhus University under the supervision of Professor Gregers Rom Andersen, working on complement. After his graduate studies Nick spent four years (2011-2015) in San Diego working as a postdoc at The Scripps Research Institute, La Jolla, CA, USA on broadly neutralizing antibodies against influenza viruses in the laboratory of Professor Ian A. Wilson. In 2016 Nick returned to Aarhus University where he now holds a position as Assistant Professor.

Nick is currently establishing his work on the role of antibodies in human health and diseases, with a special focus on antibody design.

 

Lab technician

Karen Margrethe Nielsen

Karen Margrethe obtained her degree as Laboratory Technician in 1975 from Grindstedværket (now owned by DuPont) working on enzymes applicable for laundry detergents, juice and beer. Later in 1975 Karen Margrethe got a position at Aarhus University working with X-ray crystallography in the group of late Professor Brain Clark. Since then Karen Margrethe has been working with numerous techniques including cloning, protein purification and functional assays and she is currently taking care of the mammalian cell laboratory and performs a vast amount of recombinant DNA work.

 

Post docs

 

Dennis Vestergaard Pedersen

Dennis has a master degree in Civil Engineering from Aarhus University. He obtained his PhD degree from the laboratory in 2016, studying the role of properdin in the alternative pathway of the complement system. After finishing his graduate studies, Dennis continued working in the laboratory.

Dennis experimental skills include: Stable genetic manipulation of mammalian cells and phage display. Recombinant protein expression in bacterial, yeast, baculovirus and mammalian expression systems. DNA engineering and purification of recombinant and human plasma proteins. Structural biology using X-ray crystallography and biochemical characterization using surface plasmon resonance, biolayer interferometry and flow cytometry. Functional characterization including complement deposition and hemolysis assays.

 

Janus Asbjørn Schatz-Jakobsen

Janus has a Master degree in Molecular Biology from Aarhus University and obtained his PhD degree from the laboratory in 2016 working on structural and functional description of the terminal pathway of the complement system. As a postdoc Janus has worked on the complement lectin pathway in the laboratory of Professor Steffen Thiel before returning to the lab in 2017.

Janus experimental skills include: Protein engineering and recombinant DNA work as well as expression of soluble and membrane embedded proteins using bacterial, mammalian and baculovirus expression systems. Purification of human soluble and membrane proteins of both recombinant and native source and protein structure determination using X-ray crystallography. Biochemical and functional characterization including microscale thermophoresis, cellular signaling assays, flow cytometry and TRIFMA assays.

 

PhD students

 

Trine Amalie Fogh Gadeberg

Trine has a bachelor and a master degree in Molecular Biology from Aarhus University, with 6 months of exchange at the Department of Biomedical and Molecular Sciences, Queen’s University, Ontario, Canada. Trine joined the laboratory as a project student and she was enrolled as a PhD student in 2015 focusing on structural and functional analysis of macromolecular complexes from the alternative and terminal pathways of the complement system.

Trine’s experimental skills include: Structural biology using single particle cryo and negative stain electron microscopy to describe soluble macromolecular complexes as well as SAXS, protein X-ray crystallography and structure determination. Recombinant protein expression in mammalian and bacterial systems followed by protein purification usign HPLC. DNA cloning, mutagenesis and native PAGE. Trine also uses Western blots and thermoflour assays and has performed nanobody selection by phage display.

 

Rasmus Kjeldsen Jensen

Rasmus has a bachelor and a master degree in Molecular Biology from Aarhus University. He joined the laboratory as a project student and initiated his PhD studies in 2015. Rasmus work has a special emphasis on the biology of the alternative pathway and especially C3.

Rasmus experimental skills include: Structural biology techniques including SAXS, protein crystallograhpy and single particle electron microscopy, until now primarily using negative stain electron microscopy. Mammalian cell culture. Recombinant protein expression and purification from both mammalian and bacterial cell culture. Protein purification from natural source. Protein engineering including cloning, mutagensis and other PCR based techniques. Binding kinetics using bio-layer interferometry and surface plasmon resonance.

 

Alessandra Zarantonello

Alessandra has a bachelor degree in Chemistry from the University of Padova and obtained her master degree, also in Chemistry, from Freie Universität Berlin in 2016. Later that year Alessandra joined as a PhD student working on structural and functional description of essential aspects of complement biology.

Alessandra’s experimental skills include: Recombinant protein expression and purification from mammalian and bacterial cell cultures including protein purification from human outdated plasma, DNA cloning and mutagenesis. Structural biology using negative stain electron microscopy and functional characterization using TRIFMA assay for detection of complement deposition.

 

Henrik Pedersen

Henrik has a bachelor and a master degree in Molecular Biology from Aarhus University, with 6 months of exchange at the University of Western Australia. He performed his master project in the neighbour laboratory and joined our lab as a PhD student in 2017. Henrik is working with nanobodies and complement biology.

Henrik’s experimental skills include: Recombinant protein expression using bacterial and mammalian expression systems and protein purification of recombinant and native source. Cloning and mutagenesis. Structural biology using negative stain electron microscopy and protein structure determination from X-ray crystallographic data.