Today, our lab published a comprehensive analysis of the yeast THO complex by small-angle x-ray scattering in the open access journal, PLoS One. The THO complex is intimately involved in coupling transcription to downstream mRNP processing and export from the nucleus. In the paper, we dissect the architecture of the complex through purification of sub complexes and structure determination of these by SAXS. Putting the pieces together reveals the position of each of the 5 proteins in the core complex and opens up new possibilities for a functional understanding of the complex.
For more information, have a look at the paper in PLoS One.
Poulsen J. B., Sanderson L. E., Agerschou E. D., Dedic E., Boesen T., Brodersen D. E. (2014), “Structural Characterization of the Saccharomyces cerevisiae THO Complex by Small-Angle X-Ray Scattering”, PLoS One, 9(7):e103470.